mirna mimics negative control Search Results


biotin-labeled mirna mimics or nonsense control  (Shanghai GenePharma)
90
Shanghai GenePharma biotin-labeled mirna mimics or nonsense control
Biotin Labeled Mirna Mimics Or Nonsense Control, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mir-29a mimic control (mir01101-1-5)  (Ribobio co)
90
Ribobio co mir-29a mimic control (mir01101-1-5)
Mir 29a Mimic Control (Mir01101 1 5), supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mimic and inhibitors for mir-6751-3p or mirna controls mir-6751-3p, anti-mir6751-3p, mir-nc, anti-nc  (Ribobio co)
90
Ribobio co mimic and inhibitors for mir-6751-3p or mirna controls mir-6751-3p, anti-mir6751-3p, mir-nc, anti-nc
Mimic And Inhibitors For Mir 6751 3p Or Mirna Controls Mir 6751 3p, Anti Mir6751 3p, Mir Nc, Anti Nc, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
mimic and inhibitors for mir-6751-3p or mirna controls mir-6751-3p, anti-mir6751-3p, mir-nc, anti-nc - by Bioz Stars, 2026-09
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negative control mirna duplex (mir-nc)  (Ribobio co)
90
Ribobio co negative control mirna duplex (mir-nc)
Ectopic expression <t>of</t> <t>miR-7</t> inhibits tumour growth in vitro and in vivo . ( A ) A549 and H1299 cells were transfected with miR-7 mimics and <t>miR-NC.</t> At 24, 48, and 72 h, cell proliferation was assessed by CCK-8 assay. ( B ) A549 and H1299 cells were transfected with miR-7, or miR-NC in six-well plates. Seventy-two hours later, cells were harvested, and cell cycle was analysed by FACS. ( C ) Transfected A549 and H1299 cells were replated into 6 cm dishes. Ten to fifteen days later, visible colonies were fixed and stained with crystal violet, and the colony formation ratio was calculated with the following formula: (visible colony numbers/original number of cells) × 100%. Experiments were repeated at least three times. ( D ) The effect of miR-7 on tumorigenicity was measured in the nude mice xenograft model. miR-7- and miR-NC-transfected H1299 cells (3 × 10 6 ) were injected subcutaneously into either side of the posterior flank of the same female nude mouse ( n =6). Tumour volume is shown as mean±s.e.m. All data are representative of three or two independent experiments (* P <0.05; ** P <0.01; *** P <0.001).
Negative Control Mirna Duplex (Mir Nc), supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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negative control mirna duplex (mir-nc) - by Bioz Stars, 2026-09
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mirna negative control (mir-nc)  (Shanghai GenePharma)
90
Shanghai GenePharma mirna negative control (mir-nc)
Ectopic expression <t>of</t> <t>miR-7</t> inhibits tumour growth in vitro and in vivo . ( A ) A549 and H1299 cells were transfected with miR-7 mimics and <t>miR-NC.</t> At 24, 48, and 72 h, cell proliferation was assessed by CCK-8 assay. ( B ) A549 and H1299 cells were transfected with miR-7, or miR-NC in six-well plates. Seventy-two hours later, cells were harvested, and cell cycle was analysed by FACS. ( C ) Transfected A549 and H1299 cells were replated into 6 cm dishes. Ten to fifteen days later, visible colonies were fixed and stained with crystal violet, and the colony formation ratio was calculated with the following formula: (visible colony numbers/original number of cells) × 100%. Experiments were repeated at least three times. ( D ) The effect of miR-7 on tumorigenicity was measured in the nude mice xenograft model. miR-7- and miR-NC-transfected H1299 cells (3 × 10 6 ) were injected subcutaneously into either side of the posterior flank of the same female nude mouse ( n =6). Tumour volume is shown as mean±s.e.m. All data are representative of three or two independent experiments (* P <0.05; ** P <0.01; *** P <0.001).
Mirna Negative Control (Mir Nc), supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mirna negative control (mir-nc) - by Bioz Stars, 2026-09
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mirna inhibitor negative control  (Shanghai GenePharma)
90
Shanghai GenePharma mirna inhibitor negative control
Ectopic expression <t>of</t> <t>miR-7</t> inhibits tumour growth in vitro and in vivo . ( A ) A549 and H1299 cells were transfected with miR-7 mimics and <t>miR-NC.</t> At 24, 48, and 72 h, cell proliferation was assessed by CCK-8 assay. ( B ) A549 and H1299 cells were transfected with miR-7, or miR-NC in six-well plates. Seventy-two hours later, cells were harvested, and cell cycle was analysed by FACS. ( C ) Transfected A549 and H1299 cells were replated into 6 cm dishes. Ten to fifteen days later, visible colonies were fixed and stained with crystal violet, and the colony formation ratio was calculated with the following formula: (visible colony numbers/original number of cells) × 100%. Experiments were repeated at least three times. ( D ) The effect of miR-7 on tumorigenicity was measured in the nude mice xenograft model. miR-7- and miR-NC-transfected H1299 cells (3 × 10 6 ) were injected subcutaneously into either side of the posterior flank of the same female nude mouse ( n =6). Tumour volume is shown as mean±s.e.m. All data are representative of three or two independent experiments (* P <0.05; ** P <0.01; *** P <0.001).
Mirna Inhibitor Negative Control, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mirna inhibitor negative control - by Bioz Stars, 2026-09
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mirnas and sirna transfection negative control (nc)  (Shanghai GenePharma)
90
Shanghai GenePharma mirnas and sirna transfection negative control (nc)
Ectopic expression <t>of</t> <t>miR-7</t> inhibits tumour growth in vitro and in vivo . ( A ) A549 and H1299 cells were transfected with miR-7 mimics and <t>miR-NC.</t> At 24, 48, and 72 h, cell proliferation was assessed by CCK-8 assay. ( B ) A549 and H1299 cells were transfected with miR-7, or miR-NC in six-well plates. Seventy-two hours later, cells were harvested, and cell cycle was analysed by FACS. ( C ) Transfected A549 and H1299 cells were replated into 6 cm dishes. Ten to fifteen days later, visible colonies were fixed and stained with crystal violet, and the colony formation ratio was calculated with the following formula: (visible colony numbers/original number of cells) × 100%. Experiments were repeated at least three times. ( D ) The effect of miR-7 on tumorigenicity was measured in the nude mice xenograft model. miR-7- and miR-NC-transfected H1299 cells (3 × 10 6 ) were injected subcutaneously into either side of the posterior flank of the same female nude mouse ( n =6). Tumour volume is shown as mean±s.e.m. All data are representative of three or two independent experiments (* P <0.05; ** P <0.01; *** P <0.001).
Mirnas And Sirna Transfection Negative Control (Nc), supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mirnas and sirna transfection negative control (nc) - by Bioz Stars, 2026-09
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negative control mirna  (GeneDireX Inc)
90
GeneDireX Inc negative control mirna
Ectopic expression <t>of</t> <t>miR-7</t> inhibits tumour growth in vitro and in vivo . ( A ) A549 and H1299 cells were transfected with miR-7 mimics and <t>miR-NC.</t> At 24, 48, and 72 h, cell proliferation was assessed by CCK-8 assay. ( B ) A549 and H1299 cells were transfected with miR-7, or miR-NC in six-well plates. Seventy-two hours later, cells were harvested, and cell cycle was analysed by FACS. ( C ) Transfected A549 and H1299 cells were replated into 6 cm dishes. Ten to fifteen days later, visible colonies were fixed and stained with crystal violet, and the colony formation ratio was calculated with the following formula: (visible colony numbers/original number of cells) × 100%. Experiments were repeated at least three times. ( D ) The effect of miR-7 on tumorigenicity was measured in the nude mice xenograft model. miR-7- and miR-NC-transfected H1299 cells (3 × 10 6 ) were injected subcutaneously into either side of the posterior flank of the same female nude mouse ( n =6). Tumour volume is shown as mean±s.e.m. All data are representative of three or two independent experiments (* P <0.05; ** P <0.01; *** P <0.001).
Negative Control Mirna, supplied by GeneDireX Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
negative control mirna - by Bioz Stars, 2026-09
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mirna inhibitor negative control  (Ribobio co)
90
Ribobio co mirna inhibitor negative control
Ectopic expression <t>of</t> <t>miR-7</t> inhibits tumour growth in vitro and in vivo . ( A ) A549 and H1299 cells were transfected with miR-7 mimics and <t>miR-NC.</t> At 24, 48, and 72 h, cell proliferation was assessed by CCK-8 assay. ( B ) A549 and H1299 cells were transfected with miR-7, or miR-NC in six-well plates. Seventy-two hours later, cells were harvested, and cell cycle was analysed by FACS. ( C ) Transfected A549 and H1299 cells were replated into 6 cm dishes. Ten to fifteen days later, visible colonies were fixed and stained with crystal violet, and the colony formation ratio was calculated with the following formula: (visible colony numbers/original number of cells) × 100%. Experiments were repeated at least three times. ( D ) The effect of miR-7 on tumorigenicity was measured in the nude mice xenograft model. miR-7- and miR-NC-transfected H1299 cells (3 × 10 6 ) were injected subcutaneously into either side of the posterior flank of the same female nude mouse ( n =6). Tumour volume is shown as mean±s.e.m. All data are representative of three or two independent experiments (* P <0.05; ** P <0.01; *** P <0.001).
Mirna Inhibitor Negative Control, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mirna inhibitor negative control - by Bioz Stars, 2026-09
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mirna-224 inhibitor negative control  (Shanghai GenePharma)
90
Shanghai GenePharma mirna-224 inhibitor negative control
Ectopic expression <t>of</t> <t>miR-7</t> inhibits tumour growth in vitro and in vivo . ( A ) A549 and H1299 cells were transfected with miR-7 mimics and <t>miR-NC.</t> At 24, 48, and 72 h, cell proliferation was assessed by CCK-8 assay. ( B ) A549 and H1299 cells were transfected with miR-7, or miR-NC in six-well plates. Seventy-two hours later, cells were harvested, and cell cycle was analysed by FACS. ( C ) Transfected A549 and H1299 cells were replated into 6 cm dishes. Ten to fifteen days later, visible colonies were fixed and stained with crystal violet, and the colony formation ratio was calculated with the following formula: (visible colony numbers/original number of cells) × 100%. Experiments were repeated at least three times. ( D ) The effect of miR-7 on tumorigenicity was measured in the nude mice xenograft model. miR-7- and miR-NC-transfected H1299 cells (3 × 10 6 ) were injected subcutaneously into either side of the posterior flank of the same female nude mouse ( n =6). Tumour volume is shown as mean±s.e.m. All data are representative of three or two independent experiments (* P <0.05; ** P <0.01; *** P <0.001).
Mirna 224 Inhibitor Negative Control, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mirna+mimics+negative+control/mirna+224+inhibitor+negative+control/pmc04545858-74-9-17
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negative control mirna mimics pmir-control  (GenScript corporation)
90
GenScript corporation negative control mirna mimics pmir-control
Ectopic expression <t>of</t> <t>miR-7</t> inhibits tumour growth in vitro and in vivo . ( A ) A549 and H1299 cells were transfected with miR-7 mimics and <t>miR-NC.</t> At 24, 48, and 72 h, cell proliferation was assessed by CCK-8 assay. ( B ) A549 and H1299 cells were transfected with miR-7, or miR-NC in six-well plates. Seventy-two hours later, cells were harvested, and cell cycle was analysed by FACS. ( C ) Transfected A549 and H1299 cells were replated into 6 cm dishes. Ten to fifteen days later, visible colonies were fixed and stained with crystal violet, and the colony formation ratio was calculated with the following formula: (visible colony numbers/original number of cells) × 100%. Experiments were repeated at least three times. ( D ) The effect of miR-7 on tumorigenicity was measured in the nude mice xenograft model. miR-7- and miR-NC-transfected H1299 cells (3 × 10 6 ) were injected subcutaneously into either side of the posterior flank of the same female nude mouse ( n =6). Tumour volume is shown as mean±s.e.m. All data are representative of three or two independent experiments (* P <0.05; ** P <0.01; *** P <0.001).
Negative Control Mirna Mimics Pmir Control, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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negative control mirna (scrambled)  (Merck KGaA)
90
Merck KGaA negative control mirna (scrambled)
Ectopic expression <t>of</t> <t>miR-7</t> inhibits tumour growth in vitro and in vivo . ( A ) A549 and H1299 cells were transfected with miR-7 mimics and <t>miR-NC.</t> At 24, 48, and 72 h, cell proliferation was assessed by CCK-8 assay. ( B ) A549 and H1299 cells were transfected with miR-7, or miR-NC in six-well plates. Seventy-two hours later, cells were harvested, and cell cycle was analysed by FACS. ( C ) Transfected A549 and H1299 cells were replated into 6 cm dishes. Ten to fifteen days later, visible colonies were fixed and stained with crystal violet, and the colony formation ratio was calculated with the following formula: (visible colony numbers/original number of cells) × 100%. Experiments were repeated at least three times. ( D ) The effect of miR-7 on tumorigenicity was measured in the nude mice xenograft model. miR-7- and miR-NC-transfected H1299 cells (3 × 10 6 ) were injected subcutaneously into either side of the posterior flank of the same female nude mouse ( n =6). Tumour volume is shown as mean±s.e.m. All data are representative of three or two independent experiments (* P <0.05; ** P <0.01; *** P <0.001).
Negative Control Mirna (Scrambled), supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Ectopic expression of miR-7 inhibits tumour growth in vitro and in vivo . ( A ) A549 and H1299 cells were transfected with miR-7 mimics and miR-NC. At 24, 48, and 72 h, cell proliferation was assessed by CCK-8 assay. ( B ) A549 and H1299 cells were transfected with miR-7, or miR-NC in six-well plates. Seventy-two hours later, cells were harvested, and cell cycle was analysed by FACS. ( C ) Transfected A549 and H1299 cells were replated into 6 cm dishes. Ten to fifteen days later, visible colonies were fixed and stained with crystal violet, and the colony formation ratio was calculated with the following formula: (visible colony numbers/original number of cells) × 100%. Experiments were repeated at least three times. ( D ) The effect of miR-7 on tumorigenicity was measured in the nude mice xenograft model. miR-7- and miR-NC-transfected H1299 cells (3 × 10 6 ) were injected subcutaneously into either side of the posterior flank of the same female nude mouse ( n =6). Tumour volume is shown as mean±s.e.m. All data are representative of three or two independent experiments (* P <0.05; ** P <0.01; *** P <0.001).

Journal: British Journal of Cancer

Article Title: PA28gamma emerges as a novel functional target of tumour suppressor microRNA-7 in non-small-cell lung cancer

doi: 10.1038/bjc.2013.728

Figure Lengend Snippet: Ectopic expression of miR-7 inhibits tumour growth in vitro and in vivo . ( A ) A549 and H1299 cells were transfected with miR-7 mimics and miR-NC. At 24, 48, and 72 h, cell proliferation was assessed by CCK-8 assay. ( B ) A549 and H1299 cells were transfected with miR-7, or miR-NC in six-well plates. Seventy-two hours later, cells were harvested, and cell cycle was analysed by FACS. ( C ) Transfected A549 and H1299 cells were replated into 6 cm dishes. Ten to fifteen days later, visible colonies were fixed and stained with crystal violet, and the colony formation ratio was calculated with the following formula: (visible colony numbers/original number of cells) × 100%. Experiments were repeated at least three times. ( D ) The effect of miR-7 on tumorigenicity was measured in the nude mice xenograft model. miR-7- and miR-NC-transfected H1299 cells (3 × 10 6 ) were injected subcutaneously into either side of the posterior flank of the same female nude mouse ( n =6). Tumour volume is shown as mean±s.e.m. All data are representative of three or two independent experiments (* P <0.05; ** P <0.01; *** P <0.001).

Article Snippet: The human miR-7 duplex mimic, negative control miRNA duplex (miR-NC), and miR-7 inhibitor were designed and synthesised by Ribobio Inc. (Guangzhou, Guangdong, China). miR-7 inhibitor is chemically modified, single-stranded nucleic acids designed to specifically bind to and inhibit endogenous miR-7 molecules.

Techniques: Expressing, In Vitro, In Vivo, Transfection, CCK-8 Assay, Staining, Injection

miR-7 post-transcriptionally downregulates PA28gamma expression by directly targeting its 3′-UTR. ( A ) Putative target genes were predicted by PicTar, Target Scan, and miRanda. Each circle represents one prediction algorithm with the number of predicted genes. The number listed in overlapping areas of the circles was simultaneously predicted by different algorithms. ( B ) The potential binding sequences within the 3′-UTR of PA28gamma for miR-7 is conserved in human (Hsa), chimpanzee (Ptr), rhesus (Mml), mouse (Mmu), rat, hedgehog (Eeu), shrew (Sar), horse (Eca), cow (Eta), and elephant (Dno). Seed sequences are highlighted. ( C ) The PA28gamma 3′-UTR and corresponding fragments were inserted into the region immediately downstream of the luciferase gene in the pGL3-con vector. The sequences of the predicted miR-7 binding sites within the PA28gamma 3′-UTR including the wild-type UTR or mutant UTR segments (dotted lines) are shown. ( D ) After co-transfection of miR-7 mimic or miR-NC with the above reporter plasmids and pRL-TK vector in HEK 293T cells, relative luciferase activity was analysed by Dual-Luciferase Reporter Assay. ( E ) A549 and H1299 cells were transfected with miR-7, miR-NC, PA28gamma -siRNA, and miR-7 inhibitor for 48 h. PA28gamma protein levels were examined by western blot using GAPDH as a loading control (one of three similar blots is shown). ( F ) H1299 cells were transfected with PA28gamma -siRNA, and/or miR-7 inhibitor for 72 h. PA28gamma protein levels were examined by western blot using GAPDH as a loading control (one of three similar blots is shown). These experiments were performed in triplicate and results are shown as the mean±s.e.m. (*** P <0.001).

Journal: British Journal of Cancer

Article Title: PA28gamma emerges as a novel functional target of tumour suppressor microRNA-7 in non-small-cell lung cancer

doi: 10.1038/bjc.2013.728

Figure Lengend Snippet: miR-7 post-transcriptionally downregulates PA28gamma expression by directly targeting its 3′-UTR. ( A ) Putative target genes were predicted by PicTar, Target Scan, and miRanda. Each circle represents one prediction algorithm with the number of predicted genes. The number listed in overlapping areas of the circles was simultaneously predicted by different algorithms. ( B ) The potential binding sequences within the 3′-UTR of PA28gamma for miR-7 is conserved in human (Hsa), chimpanzee (Ptr), rhesus (Mml), mouse (Mmu), rat, hedgehog (Eeu), shrew (Sar), horse (Eca), cow (Eta), and elephant (Dno). Seed sequences are highlighted. ( C ) The PA28gamma 3′-UTR and corresponding fragments were inserted into the region immediately downstream of the luciferase gene in the pGL3-con vector. The sequences of the predicted miR-7 binding sites within the PA28gamma 3′-UTR including the wild-type UTR or mutant UTR segments (dotted lines) are shown. ( D ) After co-transfection of miR-7 mimic or miR-NC with the above reporter plasmids and pRL-TK vector in HEK 293T cells, relative luciferase activity was analysed by Dual-Luciferase Reporter Assay. ( E ) A549 and H1299 cells were transfected with miR-7, miR-NC, PA28gamma -siRNA, and miR-7 inhibitor for 48 h. PA28gamma protein levels were examined by western blot using GAPDH as a loading control (one of three similar blots is shown). ( F ) H1299 cells were transfected with PA28gamma -siRNA, and/or miR-7 inhibitor for 72 h. PA28gamma protein levels were examined by western blot using GAPDH as a loading control (one of three similar blots is shown). These experiments were performed in triplicate and results are shown as the mean±s.e.m. (*** P <0.001).

Article Snippet: The human miR-7 duplex mimic, negative control miRNA duplex (miR-NC), and miR-7 inhibitor were designed and synthesised by Ribobio Inc. (Guangzhou, Guangdong, China). miR-7 inhibitor is chemically modified, single-stranded nucleic acids designed to specifically bind to and inhibit endogenous miR-7 molecules.

Techniques: Expressing, Binding Assay, Luciferase, Plasmid Preparation, Mutagenesis, Cotransfection, Activity Assay, Reporter Assay, Transfection, Western Blot, Control